Recombinant human eIF-1AX/EIF1AX protein

EIF1AX is an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. It seems to be required for maximal rate of protein biosynthesis. And it enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA (I) to 40 S ribosomal subunits. Recombinant human EIF1AX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Eukaryotic translation initiation factor 4C, Eukaryotic translation initiation factor 1A X-linked, eIF-4C, EIF4C, EIF1AP1, eIF-1A, EIF1A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

18.6 kDa (164aa) confirmed by MALDI-TOF

Additionnal Information

EIF1AX, Eukaryotic translation initiation factor 1A X-linked, Eukaryotic translation initiation factor 1A X-linked, eIF-1A, eIF-4C, EIF1A, EIF1AP1, EIF4C, ATGP0713-10 µg, ATGP0713-20 µg, ATGP0713-50 µg, ATGP0713-100 µg, ATGP0713-250 µg, ATGP0713-500 µg, ATGP0713-1 mg, ATGP0713-10, ATGP0713-20, ATGP0713-50, ATGP0713-100, ATGP0713-250, ATGP0713-500, ATGP0713-1

References & Citations

Lahn BT., et al. (1997) Science 278 (5338) :675-80.; ; Kim S., et al. (2007) Stem Cells Dev. 16 (4) :537-45.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 5mM DTT, 200 mM NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001403

Uniprot Accession Number

P47813

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MPKNKGKGGK NRRRGKNENE SEKRELVFKE DGQEYAQVIK MLGNGRLEAM CFDGVKRLCH IRGKLRKKVW INTSDIILVG LRDYQDNKAD VILKYNADEA RSLKAYGELP EHAKINETDT FGPGDDDEIQ FDDIGDDDED IDDI