Recombinant human eIF-1AX/EIF1AX protein
EIF1AX is an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA. It seems to be required for maximal rate of protein biosynthesis. And it enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA (I) to 40 S ribosomal subunits. Recombinant human EIF1AX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Eukaryotic translation initiation factor 4C, Eukaryotic translation initiation factor 1A X-linked, eIF-4C, EIF4C, EIF1AP1, eIF-1A, EIF1A
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
18.6 kDa (164aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Lahn BT., et al. (1997) Science 278 (5338) :675-80.; ; Kim S., et al. (2007) Stem Cells Dev. 16 (4) :537-45.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 5mM DTT, 200 mM NaCl
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001403
Uniprot Accession Number
P47813
Species
Human
AA Sequence
Frequently Asked Questions
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