Recombinant human ECH1 protein
ECH1 (enoyl coenzyme A hydratase 1) belongs to the hydratase/isomerase superfamily. This Protein shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. It contains a C-terminal peroxisomal targeting sequence, localizes to both the peroxisome and the mitochondria. This enzyme involved in the auxiliary step of the fatty acid beta-oxidation pathway specifically functioning to catalyze the isomerization of 3-trans, 5-cis-dienoyl-CoA to 2-trans, 4-transdienoyl-CoA. Recombinant human ECH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Enoyl Coenzyme A hydratase 1 peroxisomal, enoyl Coenzyme A hydratase 1 peroxisomal, HPXEL
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
34.4 kDa (316aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Goehler H., et al. (2004) Mol. Cell 15 (6) :853-65.; ; FitzPatrick DR., et al. (1995) Genomics 27 (3) :457-66.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
AAH17408
Uniprot Accession Number
Q13011
Species
Human
AA Sequence
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