Recombinant human UBE2R1/CDC34 protein

CDC34 is a member of the ubiquitin-conjugating enzyme family. ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. CDC34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae CDC34, which is essential for the G1 to S phase transition. Recombinant human CDC34 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cell division cycle 34, Ubiqiutin conjugating enzyme, Ubiquitin-conjugating enzyme E2 R1, E2 ubiquitin-conjugating enzyme R1, Ubiquitin-conjugating enzyme E2-32 kDa complementing, Ubiquitin-conjugating enzyme E2-CDC34, Ubiquitin-protein ligase R1, E2-CDC34, UBE2R1, UBC3, UBCH3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

28.9 kDa (256aa) confirmed by MALDI-TOF

Additionnal Information

UbcH3, Cdc34, ubiquitin-conjugating enzyme E2 R1, E2-CDC34, uBC3, uBCH3, uBE2R1, ATGP0710-10 µg, ATGP0710-20 µg, ATGP0710-50 µg, ATGP0710-100 µg, ATGP0710-250 µg, ATGP0710-500 µg, ATGP0710-1 mg, ATGP0710-10, ATGP0710-20, ATGP0710-50, ATGP0710-100, ATGP0710-250, ATGP0710-500, ATGP0710-1

References & Citations

Sadowski M., et al. (2007) Biochem. J. 405:569-581; ; Gazdoiu S., et al. (2005) Proc Natl Acad Sci u S A 102 (42) :15053-8.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_004350

Uniprot Accession Number

P49427

Species

Human

AA Sequence

MARPLVPSSQ KALLLELKGL QEEPVEGFRV TLVDEGDLYN WEVAIFGPPN TYYEGGYFKA RLKFPIDYPY SPPAFRFLTK MWHPNIYETG DVCISILHPP VDDPQSGELP SERWNPTQNV RTILLSVISL LNEPNTFSPA NVDASVMYRK WKESKGKDRE YTDIIRKQVL GTKVDAERDG VKVPTTLAEY CVKTKAPAPD EGSDLFYDDY YEDGEVEEEA DSCFGDDEDD SGTEES