Recombinant human ADAT2 protein

Adenosine deaminase (ADA), also known as ADAT2 is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. Also, ADAT2 is thought to participate in the deamination of adenosine-34 to inosine in many tRNAs. Belonging to the cytidine and deoxycytidylate deaminase protein family, ADAT2 employs zinc as a cofactor. ADAT2 is a 191 amino acid protein that exists as two isoforms produced by alternative splicing events. Recombinant human ADAT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

TRNA-specific adenosine deaminase 2, DEADC1, TAD2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

23.2 kDa (211aa) confirmed by MALDI-TOF

Additionnal Information

ADAT2, tRNA-specific adenosine deaminase 2, DEADC1, TAD2, ATGP0709-10 µg, ATGP0709-20 µg, ATGP0709-50 µg, ATGP0709-100 µg, ATGP0709-250 µg, ATGP0709-500 µg, ATGP0709-1 mg, ATGP0709-10, ATGP0709-20, ATGP0709-50, ATGP0709-100, ATGP0709-250, ATGP0709-500, ATGP0709-1

References & Citations

Persico AM, et al. (2000) Am. J. Med. Genet. 96 (6) : 784-90.; ; Kimura, K., et al. (2006) Genome Res. 16: 55-65.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 50 mM NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_872309

Uniprot Accession Number

Q7Z6V5

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MEAKAAPKPA ASGACSVSAE ETEKWMEEAM HMAKEALENT EVPVGCLMVY NNEVVGKGRN EVNQTKNATR HAEMVAIDQV LDWCRQSGKS PSEVFEHTVL YVTVEPCIMC AAALRLMKIP LVVYGCQNER FGGCGSVLNI ASADLPNTGR PFQCIPGYRA EEAVEMLKTF YKQENPNAPK SKVRKKECQK S