Recombinant human UPP1 protein

Uridine phosphorylase 1, also known as uPP1 catalyses the reversible phosphorolysis of uridine to uracil. The reaction products are then utilized as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis. The expression levels and the enzymatic activity of uPP1 are higher in human solid tumors than in adjacent normal tissues. In addition, uPP1 controls the homeostatic regulation of uridine concentration in plasma and tissues and plays a role in the intracellular activation of 5-fluorouracil. Recombinant human uPP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Uridine phosphorylase 1, uP, uPASE, uPP, urdPase 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36 kDa (330aa) confirmed by MALDI-TOF

Additionnal Information

UPP1, uridine phosphorylase 1, uP, uPASE, uPP, urdPase 1, ATGP0707-10 µg, ATGP0707-20 µg, ATGP0707-50 µg, ATGP0707-100 µg, ATGP0707-250 µg, ATGP0707-500 µg, ATGP0707-1 mg, ATGP0707-10, ATGP0707-20, ATGP0707-50, ATGP0707-100, ATGP0707-250, ATGP0707-500, ATGP0707-1

References & Citations

Kanzaki A., et al. (2002) Int J Cancer. 97 (5) :631-5.; ; Russll RL., et al. (2001) J Biol Chem. 276 (16) :13302-7.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 200 mM NaCl

Scientific Category

Cancer

NCBI Accession Number

NP_853628

Uniprot Accession Number

Q16831

Species

Human

AA Sequence

MAATGANAEK AESHNDCPVR LLNPNIAKMK EDILYHFNLT TSRHNFPALF GDVKFVCVGG SPSRMKAFIR CVGAELGLDC PGRDYPNICA GTDRYAMYKV GPVLSVSHGM GIPSISIMLH ELIKLLYYAR CSNVTIIRIG TSGGIGLEPG TVVITEQAVD TCFKAEFEQI VLGKRVIRKT DLNKKLVQEL LLCSAELSEF TTVVGNTMCT LDFYEGQGRL DGALCSYTEK DKQAYLEAAY AAGVRNIEME SSVFAAMCSA CGLQAAVVCV TLLNRLEGDQ ISSPRNVLSE YQQRPQRLVS YFIKKKLSKA