Recombinant human QPRT protein

Quinolinate phosphoribosyltransferase, also known as QPRT, is a major enzyme in the catabolism of quinolinate. It is an intermediate in the tryptophannicotinamide adenine dinucleotide (NAD) pathway, leading to the production of nicotinic acid, carbon dioxide and pyrophosphate. Elevation of QPRT levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease. Recombinant human QPRT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Nicotinate nucleotide pyrophosphorylase (carboxylating), Nicotinate nucleotide pyrophosphorylase carboxylating, Quinolinate phosphoribosyltransferase, QPRTase, QAPRTase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

32.9 kDa (317aa) confirmed by MALDI-TOF

Additionnal Information

QPRT, Nicotinate nucleotide pyrophosphorylase (carboxylating), Nicotinate nucleotide pyrophosphorylase carboxylating, Quinolinate phosphoribosyltransferase, QPRTase, QAPRTase, ATGP0706-10 µg, ATGP0706-20 µg, ATGP0706-50 µg, ATGP0706-100 µg, ATGP0706-250 µg, ATGP0706-500 µg, ATGP0706-1 mg, ATGP0706-10, ATGP0706-20, ATGP0706-50, ATGP0706-100, ATGP0706-250, ATGP0706-500, ATGP0706-1

References & Citations

Delaney J., et al. (2005) Arch Toxicol. 79 (2) :208-223.; ; Eads J C., et al. (1997) Structure. 5:47-58.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_055113

Uniprot Accession Number

Q15274

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MDAEGLALLL PPVTLAALVD SWLREDCPGL NYAALVSGAG PSQAALWAKS PGVLAGQPFF DAIFTQLNCQ VSWFLPEGSK LVPVARVAEV RGPAHCLLLG ERVALNTLAR CSGIASAAAA AVEAARGAGW TGHVAGTRKT TPGFRLVEKY GLLVGGAASH RYDLGGLVMV KDNHVVAAGG VEKAVRAARQ AADFALKVEV ECSSLQEAVQ AAEAGADLVL LDNFKPEELH PTATVLKAQF PSVAVEASGG ITLDNLPQFC GPHIDVISMG MLTQAAPALD FSLKLFAKEV APVPKIH