Recombinant human GMPR2 protein

Guanosine monophosphate reductase 2, also known as GMPR2, is the only known metabolic step by which guanine nucleotides can be converted to the pivotal precursor of both adenine and guanine nucleotides. GMPR2 catalyzes the irreversible and NADPH-dependent reductive deamination of GMP to IMP, and plays a critical role in re-utilization of free intracellular bases and purine nucleosides. Recombinant human GMPR2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Guanosine monophosphate reductase 2, GMP reductase 2, Guanosine 5'-monophosphate oxidoreductase 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

40 kDa (368aa) confirmed by MALDI-TOF

Additionnal Information

GMPR2, Guanosine monophosphate reductase 2, GMP reductase 2, Guanosine 5'-monophosphate oxidoreductase 2, ATGP0704-10 µg, ATGP0704-20 µg, ATGP0704-50 µg, ATGP0704-100 µg, ATGP0704-250 µg, ATGP0704-500 µg, ATGP0704-1 mg, ATGP0704-10, ATGP0704-20, ATGP0704-50, ATGP0704-100, ATGP0704-250, ATGP0704-500, ATGP0704-1

References & Citations

Li J., et al. (2006) J Mol Biol. 355 (5) :980-8.; ; Denq Y., et al. (2002) Int J Biochem Cell Biol. 34 (9) :1035-50.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001002002

Uniprot Accession Number

Q9P2T1

Species

Human

AA Sequence

MPHIDNDVKL DFKDVLLRPK RSTLKSRSEV DLTRSFSFRN SKQTYSGVPI IAANMDTVGT FEMAKVLCKF SLFTAVHKHY SLVQWQEFAG QNPDCLEHLA ASSGTGSSDF EQLEQILEAI PQVKYICLDV ANGYSEHFVE FVKDVRKRFP QHTIMAGNVV TGEMVEELIL SGADIIKVGI GPGSVCTTRK KTGVGYPQLS AVMECADAAH GLKGHIISDG GCSCPGDVAK AFGAGADFVM LGGMLAGHSE SGGELIERDG KKYKLFYGMS SEMAMKKYAG GVAEYRASEG KTVEVPFKGD VEHTIRDILG GIRSTCTYVG AAKLKELSRR TTFIRVTQQV NPIFSEAC