Recombinant human ECHS1 protein
Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Enoyl Coenzyme A hydratase short chain 1 mitochondrial, Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, SCEH
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
30.6 kDa (284aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kanazawa M., et al. (1993) Enzyme Protein. 47:9-13; ; Stern J R., et al. (1956) J Biol Chem. 218:985-1002.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
AAH08906
Uniprot Accession Number
P30084
Species
Human
AA Sequence
Frequently Asked Questions
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