Recombinant human GNPDA1 protein
Glucosamine-6-phosphate isomerase 1, also known as GNPDA1, catalyzes the conversion of glucosamine-6-phosphate to fructose-6-phosphate, a reaction that under physiological conditions proceeds to the formation of fructose-6-phosphate. It exist is ubiquitously expressed with highest expression in testes, ovary and heart. In addition, GNPDA1 triggers calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo. Recombinant human GNPDA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glucosamine-6-phosphate isomerase 1, GNP1, GNPDA, GNPI, GPI, HLN, Oscillin
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
34.8 kDa (309aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Wolosker H., et al. (1998) FASEB J. 12 (1) :91-9.; ; Montag M., et al. (1999) FEBS Lett.458:141-144.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 50 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_005462
Uniprot Accession Number
P46926
Species
Human
AA Sequence
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