Recombinant human GNPDA1 protein

Glucosamine-6-phosphate isomerase 1, also known as GNPDA1, catalyzes the conversion of glucosamine-6-phosphate to fructose-6-phosphate, a reaction that under physiological conditions proceeds to the formation of fructose-6-phosphate. It exist is ubiquitously expressed with highest expression in testes, ovary and heart. In addition, GNPDA1 triggers calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo. Recombinant human GNPDA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Glucosamine-6-phosphate isomerase 1, GNP1, GNPDA, GNPI, GPI, HLN, Oscillin

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.8 kDa (309aa) confirmed by MALDI-TOF

Additionnal Information

GNPDA1, Glucosamine-6-phosphate isomerase 1, GNP1, GNPDA, GNPI, GPI, HLN, Oscillin, ATGP0701-10 µg, ATGP0701-20 µg, ATGP0701-50 µg, ATGP0701-100 µg, ATGP0701-250 µg, ATGP0701-500 µg, ATGP0701-1 mg, ATGP0701-10, ATGP0701-20, ATGP0701-50, ATGP0701-100, ATGP0701-250, ATGP0701-500, ATGP0701-1

References & Citations

Wolosker H., et al. (1998) FASEB J. 12 (1) :91-9.; ; Montag M., et al. (1999) FEBS Lett.458:141-144.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 50 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_005462

Uniprot Accession Number

P46926

Species

Human

AA Sequence

MKLIILEHYS QASEWAAKYI RNRIIQFNPG PEKYFTLGLP TGSTPLGCYK KLIEYYKNGD LSFKYVKTFN MDEYVGLPRD HPESYHSFMW NNFFKHIDIH PENTHILDGN AVDLQAECDA FEEKIKAAGG IELFVGGIGP DGHIAFNEPG SSLVSRTRVK TLAMDTILAN ARFFDGELTK VPTMALTVGV GTVMDAREVM ILITGAHKAF ALYKAIEEGV NHMWTVSAFQ QHPRTVFVCD EDATLELKVK TVKYFKGLML VHNKLVDPLY SIKEKETEKS QSSKKPYSD