Recombinant human RCL protein

Deoxyribonucleoside 5'-monophosphate N-glycosidase isoform 1, also known as RCL, belonged to the N-deoxyribosyltransferase family. This protein catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. RCL was identified on the basis of its stimulation by c-Myc protein. The latter is a transcription factor that participates in the regulation of cell proliferation, differentiation, and apoptosis. Recombinant human RCL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Deoxyribonucleoside 5'-monophosphate N-glycosidase isoform 1, c-Myc-responsive protein Rcl, C6orf108

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

21.2 kDa (194aa) confirmed by MALDI-TOF

Additionnal Information

RCL, Deoxyribonucleoside 5'-monophosphate N-glycosidase isoform 1, c-Myc-responsive protein Rcl, C6orf108, ATGP0698-10 µg, ATGP0698-20 µg, ATGP0698-50 µg, ATGP0698-100 µg, ATGP0698-250 µg, ATGP0698-500 µg, ATGP0698-1 mg, ATGP0698-10, ATGP0698-20, ATGP0698-50, ATGP0698-100, ATGP0698-250, ATGP0698-500, ATGP0698-1

References & Citations

Ghiorghi Y K., et al. (2007) J Biol Chem. 282 (11) :8150-6.; ; Munqall A J., et al. (2003) Nature. 425 (6960) :805-11.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_006434

Uniprot Accession Number

O43598

Species

Human

AA Sequence

MAAAMVPGRS ESWERGEPGR PALYFCGSIR GGREDRTLYE RIVSRLRRFG TVLTEHVAAA ELGARGEEAA GGDRLIHEQD LEWLQQADVV VAEVTQPSLG VGYELGRAVA FNKRILCLFR PQSGRVLSAM IRGAADGSRF QVWDYEEGEV EALLDRYFEA DPPGQVAASP DPTT