Recombinant human RABIF protein
RABIF, also known as guanine nucleotide exchange factor MSS4, is a guanine-nucleotide-releasing protein that may participate in vesicular transport. This protein stimulates GTP-GDP exchange in Sec4 and Rab and binds to a subset of genetically related Rab proteins. Recombinant human RABIF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Guanine nucleotide exchange factor MSS4, MSS4, RASGFR3, RASGRF3, Rab-interacting factor, Guanine nucleotide exchange factor MSS4
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
16 kDa (143aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Muller-Pillasch F, et al.. (1998) Genomics 46 (3) : 389-96; ; Yu H, et al. (1995) Nature 376 (6543) : 788-91;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_002862
Uniprot Accession Number
P47224
Species
Human
AA Sequence
MEPAEQPSELVSAEGRNRKA VLCQRCGSRV LQPGTALFSR RQLFLPSMRK KPALSDGSNP DGDLLQEHWL VEDMFIFENV GFTKDVGNIK FLVCADCEIG PIGWHCLDDK NSFYVALERV SHE
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