Recombinant human Guanylate kinase 1/GUK1 protein
GuK1, also known as GMK, belongs to the guanylate kinase family. This protein exists as a monomer that catalyzes the ATP-dependent conversion of GMP to GDP, thereby playing an essential role in the recycling of GMP. Via its catalytic activity, GuK1 is thought to participate in regulating the supply of guanine nucleotides to signal transduction pathways. Overexpression of GuK1 is associated with pituitary adenocarcinomas, suggesting that GuK1 is involved in tumorigenesis. Recombinant human GuK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Guanylate kinase, GMK, GMP kinase, GMPK
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
23.9 kDa (217aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Da Rocha AA., et al. (2006) Pituitary. 9 (2) :83-92. ; ; Brady WA., et al. (1996) J Biol Chem. 271 (28) :16734-40.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.
Scientific Category
Cancer
NCBI Accession Number
NP_000849
Uniprot Accession Number
Q16774
Species
Human
AA Sequence
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