Recombinant human Galectin-8/LGALS8 protein

LGALS8, also known as prostate-specific membrane antigen (PCTA1), is an additional prostate-specific antigen that is overexpressed only in malignant tumors and therefore is a more specific identifier of malignancies. It is a member of the galectin gene family which mediates both cell-cell and cell matrix interactions in a manner similar to the selectin subgroup of C-type lectins. Recombinant human LGALS8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Measured by its ability to agglutinate human red blood cells. The ED50 range ≤ 10 µg/mL.

Product Name Alternative

Prostate carcinoma tumor antigen 1, Po66-CBP, Po66 carbohydrate-binding protein, PCTA1, Galectin-8, Gal-8

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by its ability to agglutinate human red blood cells. The ED50 range ≤ 10 µg/mL.

Molecular Weight

37.9 kDa (337aa) confirmed by MALDI-TOF

Additionnal Information

Galectin8, Galectin-8, Gal-8, PCTA1, Po66-CBP, Prostate carcinoma tumor antigen 1, Galectin-8, ATGP0688-10 µg, ATGP0688-20 µg, ATGP0688-50 µg, ATGP0688-100 µg, ATGP0688-250 µg, ATGP0688-500 µg, ATGP0688-1 mg, ATGP0688-10, ATGP0688-20, ATGP0688-50, ATGP0688-100, ATGP0688-250, ATGP0688-500, ATGP0688-1

References & Citations

Kobayashi T., et al. (2010) Leukemia. 24 (4) :843-50.; ; Streetly MJ., et al. (2010) Blood. 115 (19) :3939-48.;

Other References

Suzuki O et al., Sialylation by B-galactoside a-2,6-sialyltransferase and N-glycans regulate cell adhesion and invasion in human anaplastic large cell lymphoma. (Int J Oncol. 2015) {https://pubmed.ncbi.nlm.nih.gov/25573487/}; ; Campanero-Rhodes, M.A, et al. Exploration of Galectin Ligands Displayed on Gram-Negative Respiratory Bacterial Pathogens with Different Cell Surface Architectures. (Biomolecules. 2021) {https://pubmed.ncbi.nlm.nih.gov/33919637/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M Nacl, 1mM DTT, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_963837

Uniprot Accession Number

O00214

Species

Human

AA Sequence

MMLSLNNLQN IIYNPVIPFV GTIPDQLDPG TLIVIRGHVP SDADRFQVDL QNGSSMKPRA DVAFHFNPRF KRAGCIVCNT LINEKWGREE ITYDTPFKRE KSFEIVIMVL KDKFQVAVNG KHTLLYGHRI GPEKIDTLGI YGKVNIHSIG FSFSSDLQST QASSLELTEI SRENVPKSGT PQLRLPFAAR LNTPMGPGRT VVVKGEVNAN AKSFNVDLLA GKSKDIALHL NPRLNIKAFV RNSFLQESWG EEERNITSFP FSPGMYFEMI IYCDVREFKV AVNGVHSLEY KHRFKELSSI DTLEINGDIH LLEVRSW