Recombinant human STAT1 protein

STAT1, also known as STAT91, is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 is activated by peptide hormones, growth factors and cytokines, particularly cytokines of the interferon family. Recombinant human STAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Signal transducer and activator of transcription 1 91kDa, ISGF-3, STAT91, Transcription factor ISGF-3 components p91/p84, Signal transducer and activator of transcription 1, 91kDa

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

85.2 kDa (732aa) confirmed by MALDI-TOF

Additionnal Information

STAT1, Signal transducer and activator of transcription 1 91kDa, ISGF-3, STAT91, Transcription factor ISGF-3 components p91/p84, Signal transducer and activator of transcription 1, 91kDa, ATGP0685-10 µg, ATGP0685-20 µg, ATGP0685-50 µg, ATGP0685-100 µg, ATGP0685-250 µg, ATGP0685-500 µg, ATGP0685-1 mg, ATGP0685-10, ATGP0685-20, ATGP0685-50, ATGP0685-100, ATGP0685-250, ATGP0685-500, ATGP0685-1

References & Citations

Farlik M., et al. (2010) Immunity. 33 (1) :25-34.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M Nacl, 1mM DTT, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_644671.1

Uniprot Accession Number

P42224

Species

Human

AA Sequence

MSQWYELQQL DSKFLEQVHQ LYDDSFPMEI RQYLAQWLEK QDWEHAANDV SFATIRFHDL LSQLDDQYSR FSLENNFLLQ HNIRKSKRNL QDNFQEDPIQ MSMIIYSCLK EERKILENAQ RFNQAQSGNI QSTVMLDKQK ELDSKVRNVK DKVMCIEHEI KSLEDLQDEY DFKCKTLQNR EHETNGVAKS DQKQEQLLLK KMYLMLDNKR KEVVHKIIEL LNVTELTQNA LINDELVEWK RRQQSACIGG PPNACLDQLQ NWFTIVAESL QQVRQQLKKL EELEQKYTYE HDPITKNKQV LWDRTFSLFQ QLIQSSFVVE RQPCMPTHPQ RPLVLKTGVQ FTVKLRLLVK LQELNYNLKV KVLFDKDVNE RNTVKGFRKF NILGTHTKVM NMEESTNGSL AAEFRHLQLK EQKNAGTRTN EGPLIVTEEL HSLSFETQLC QPGLVIDLET TSLPVVVISN VSQLPSGWAS ILWYNMLVAE PRNLSFFLTP PCARWAQLSE VLSWQFSSVT KRGLNVDQLN MLGEKLLGPN ASPDGLIPWT RFCKENINDK NFPFWLWIES ILELIKKHLL PLWNDGCIMG FISKERERAL LKDQQPGTFL LRFSESSREG AITFTWVERS QNGGEPDFHA VEPYTKKELS AVTFPDIIRN YKVMAAENIP ENPLKYLYPN IDKDHAFGKY YSRPKEAPEP MELDGPKGTG YIKTELISVS EV