Recombinant human TPM1 protein
TPM1 is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. TPM1 is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. Recombinant human TPM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Tropomyosin alpha-1 chain, TPM1, CMD1Y, HTM-alpha, TMSA, Tropomyosin-1, Alpha-tropomyosin, Tropomyosin alpha-1 chain
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by BCA assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
35.0 kDa (304aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher) )
Additionnal Information
References & Citations
Lees-Miller JP.. (1991) Bioessays. 13 (9) :429-37.; ; Perry SV. (2001) J Muscle Res Cell Motil. 22 (1) :5-49.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl,1mM EDTA, 0.1mM PMSF
Scientific Category
Cytoskeleton
NCBI Accession Number
NP_000357.3
Uniprot Accession Number
P09493
Species
Human
AA Sequence
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