Recombinant human TPM1 protein

TPM1 is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. TPM1 is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. Recombinant human TPM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tropomyosin alpha-1 chain, TPM1, CMD1Y, HTM-alpha, TMSA, Tropomyosin-1, Alpha-tropomyosin, Tropomyosin alpha-1 chain

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by BCA assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

35.0 kDa (304aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher) )

Additionnal Information

TPM1, Tropomyosin alpha-1 chain, TPM1, CMD1Y, HTM-alpha, TMSA, Tropomyosin-1, Alpha-tropomyosin, Tropomyosin alpha-1 chain, ATGP0683-10 µg, ATGP0683-20 µg, ATGP0683-50 µg, ATGP0683-100 µg, ATGP0683-250 µg, ATGP0683-500 µg, ATGP0683-1 mg, ATGP0683-10, ATGP0683-20, ATGP0683-50, ATGP0683-100, ATGP0683-250, ATGP0683-500, ATGP0683-1

References & Citations

Lees-Miller JP.. (1991) Bioessays. 13 (9) :429-37.; ; Perry SV. (2001) J Muscle Res Cell Motil. 22 (1) :5-49.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl,1mM EDTA, 0.1mM PMSF

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_000357.3

Uniprot Accession Number

P09493

Species

Human

AA Sequence

MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEDRSKQLE DELVSLQKKL KGTEDELDKY SEALKDAQEK LELAEKKATD AEADVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA DESERGMKVI ESRAQKDEEK MEIQEIQLKE AKHIAEDADR KYEEVARKLV IIESDLERAE ERAELSEGQV RQLEEQLRIM DQTLKALMAA EDKYSQKEDR YEEEIKVLSD KLKEAETRAE FAERSVTKLE KSIDDLEDEL YAQKLKYKAI SEELDHALND MTSM