Recombinant human GMF-beta protein
GMFB, also known as GMF, belongs to the GMF subfamily of the larger actin-binding protein ADF family. This protein, which is phosphorylated following phorbol ester stimulation, is important for the nervous system. It causes brain cell differentiation, stimulates neural regeneration and inhibits tumor cell proliferation. Overexpression of GMFB in astrocytes has been shown to enhance brain-derived neurotrohic factor (BDNF) production. GMFB expression is increased by exercise, and the protein is crucial for exercise-induction of BDNF. Recombinant human GMFB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Glia maturation factor beta, GMF, GMF beta, Glia maturation factor beta
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
18.8 kDa (162aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Li YL., et al. (2010) Eur J Cancer. 46 (11) :2104-18.; ; Wada T., et al. (2009) Int J Oncol. 35 (5) :973-6.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_004115
Uniprot Accession Number
P60983
Species
Human
AA Sequence
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