Recombinant human Malate dehydrogenase 2/MDH2 protein

MDH2 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH2 is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. It is widely expressed with high expression levels found in adrenal, small intestine, heart and pancreas. Recombinant human MDH2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 700 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Product Name Alternative

Malate dehydrogenase 2 NAD (mitochondrial), M-MDH, MDH, MOR1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 700unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Molecular Weight

35.2 kDa (335aa) confirmed by MALDI-TOF

Additionnal Information

MDH2, Malate dehydrogenase 2 NAD (mitochondrial), M-MDH, MDH, MOR1, Malate dehydrogenase 2, NAD (mitochondrial), ATGP0679-10 µg, ATGP0679-20 µg, ATGP0679-50 µg, ATGP0679-100 µg, ATGP0679-250 µg, ATGP0679-500 µg, ATGP0679-1 mg, ATGP0679-10, ATGP0679-20, ATGP0679-50, ATGP0679-100, ATGP0679-250, ATGP0679-500, ATGP0679-1

References & Citations

Thummel CS., et al. (2010) Dev Dyn. 239 (3) :954-64; ; Lee JS., et al (2002) J Basic Microbiol. 42 (4) :238-45.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_005909

Uniprot Accession Number

P40926

Species

Human

AA Sequence

AKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL TALTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVKSQET ECTYFSTPLL LGKKGIEKNL GIGKVSSFEE KMISDAIPEL KASIKKGEDF VKTLK