Recombinant human MMAB protein

MMAB is a protein that catalyzes the final step in the conversion of vitamin B (12) into adenosylcobalamin (AdoCbl), a vitamin B12 containing coenzyme for methylmalonyl-CoA mutase (MCM) . Impaired MMAB activity leads to the inherited disorder vitamin B12 dependent methylmalonic aciduria linked to the cblB complementation group. Recombinant human MMAB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Methylmalonic aciduria (cobalamin deficiency) cblB type, ATR, ATP:cob (I) alamin adenosyltransferase, Methylmalonic aciduria (cobalamin deficiency) cblB type

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

26.3 kDa (239aa) confirmed by MALDI-TOF

Additionnal Information

MMAB, Methylmalonic aciduria (cobalamin deficiency) cblB type, ATR, ATP:cob (I) alamin adenosyltransferase, Methylmalonic aciduria (cobalamin deficiency) cblB type, ATGP0677-10 µg, ATGP0677-20 µg, ATGP0677-50 µg, ATGP0677-100 µg, ATGP0677-250 µg, ATGP0677-500 µg, ATGP0677-1 mg, ATGP0677-10, ATGP0677-20, ATGP0677-50, ATGP0677-100, ATGP0677-250, ATGP0677-500, ATGP0677-1

References & Citations

Gravel RA., et al. (2009) Mol Genet Metab. 98 (3) :278-84; ; Edwards AM., et al (2004) J Biol Chem. 279 (22) :23646-53.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

AAH05054

Uniprot Accession Number

Q96EY8

Species

Human

AA Sequence

MQSRGPQGVE DGDRPQPSSK TPRIPKIYTK TGDKGFSSTF TGERRPKDDQ VFEAVGTTDE LSSAIGFALE LVTEKGHTFA EELQKIQCTL QDVGSALATP CSSAREAHLK YTTFKAGPIL ELEQWIDKYT SQLPPLTAFI LPSGGKISSA LHFCRAVCRR AERRVVPLVQ MGETDANVAK FLNRLSDYLF TLARYAAMKE GNQEKIYKKN DPSAESEGL