Recombinant human THTPA protein

THTPA, also known as THTP or THTPASE, is a member of the THTPase family. This protein is localized to the cytoplasm and expressed at low levels in a variety of tissues, including testis, uterus, prostate, bladder, lung and kidney. THTPA is a hydrolase that catalyzes the H2O-dependent hydrolysis of thiamine triphosphate (THTP) to thiamine diphosphate (THDP), the major form of thiamine within the cell. THTPA exists as a monomer and functions at an optimal pH of 8. 5. Recombinant human THTPA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Thiamine-triphosphatase, MGC2652, THTP, THTPASE, Thiamine-triphosphatase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.7 kDa (250aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

THTPA, Thiamine-triphosphatase, MGC2652, THTP, THTPASE, Thiamine-triphosphatase, ATGP0676-10 µg, ATGP0676-20 µg, ATGP0676-50 µg, ATGP0676-100 µg, ATGP0676-250 µg, ATGP0676-500 µg, ATGP0676-1 mg, ATGP0676-10, ATGP0676-20, ATGP0676-50, ATGP0676-100, ATGP0676-250, ATGP0676-500, ATGP0676-1

References & Citations

Lakaye B., et al. (2002) J Biol Chem. 277 (16) :13771-7. ; ; Lakaye B., et al. (2004) Int J Biochem Cell Biol. 36 (7) :1348-64.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001119811

Uniprot Accession Number

Q9BU02

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAQGLIEVER KFLPGPGTEE RLQELGGTLE YRVTFRDTYY DTPELSLMQA DHWLRRREDS GWELKCPGAA GVLGPHTEYK ELTAEPTIVA QLCKVLRADG LGAGDVAAVL GPLGLQEVAS FVTKRSAWKL VLLGADEEEP QLRVDLDTAD FGYAVGEVEA LVHEEAEVPT ALEKIHRLSS MLGVPAQETA PAKLIVYLQR FRPQDYQRLL EVNSSRERPQ ETEDPDHCLG