Recombinant human ACAA protein

ACAA1 is a member of the thiolase family of enzymes and is involved in lipid metabolism. This protein is localized to the peroxisome and catalyzes the conversion of acyl-CoA and acetyl-CoA to 3-oxoacyl-CoA in the fatty acid oxidation pathway. ACAA1 shows high enzymatic activity in liver, kidney, intestine and white adipose tissue in rats. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Recombinant human ACAA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

3-ketoacyl-CoA thiolase peroxisomal, ACAA, PTHIO, THIO, 3-ketoacyl-CoA thiolase, peroxisomal

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

43.8 kDa (419aa) confirmed by MALDI-TOF

Additionnal Information

ACAA, 3-ketoacyl-CoA thiolase peroxisomal, ACAA, PTHIO, THIO, 3-ketoacyl-CoA thiolase, peroxisomal, ATGP0674-10 µg, ATGP0674-20 µg, ATGP0674-50 µg, ATGP0674-100 µg, ATGP0674-250 µg, ATGP0674-500 µg, ATGP0674-1 mg, ATGP0674-10, ATGP0674-20, ATGP0674-50, ATGP0674-100, ATGP0674-250, ATGP0674-500, ATGP0674-1

References & Citations

Bout A., et al. (1991) Biochim Biophys Acta. 1090 (1) :43-51. ; ; Patel S., et al. (2003) Eur Respir J. 22 (5) :755-60.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT, 0.1M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001598

Uniprot Accession Number

P09110

Species

Human

AA Sequence

MLSGAPQASA ADVVVVHGRR TAICRAGRGG FKDTTPDELL SAVMTAVLKD VNLRPEQLGD ICVGNVLQPG AGAIMARIAQ FLSDIPETVP LSTVNRQCSS GLQAVASIAG GIRNGSYDIG MACGVESMSL ADRGNPGNIT SRLMEKEKAR DCLIPMGITS ENVAERFGIS REKQDTFALA SQQKAARAQS KGCFQAEIVP VTTTVHDDKG TKRSITVTQD EGIRPSTTME GLAKLKPAFK KDGSTTAGNS SQVSDGAAAI LLARRSKAEE LGLPILGVLR SYAVVGVPPD IMGIGPAYAI PVALQKAGLT VSDVDIFEIN EAFASQAAYC VEKLRLPPEK VNPLGGAVAL GHPLGCTGAR QVITLLNELK RRGKRAYGVV SMCIGTGMGA AAVFEYPGN