Recombinant human RCN1 protein

Reticulocalbin 1 (RCN1) is a calcium-binding protein. It binds calcium and may regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. The protein contains six conserved regions with similarity to a high affinity Ca (+2) -binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane. Recombinant human RCN1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Reticulocalbin-1, PIG20, RCAL, RCN, Reticulocalbin-1, Reticulocalbin 1, Reticulocalbin1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

40.4 kDa (341aa) confirmed by MALDI-TOF (Real Molecular weight on SDS-PAGE will be shift up)

Additionnal Information

RCN1, Reticulocalbin-1, PIG20, RCAL, RCN, Reticulocalbin-1, Reticulocalbin 1, Reticulocalbin1, ATGP0672-10 µg, ATGP0672-20 µg, ATGP0672-50 µg, ATGP0672-100 µg, ATGP0672-250 µg, ATGP0672-500 µg, ATGP0672-1 mg, ATGP0672-10, ATGP0672-20, ATGP0672-50, ATGP0672-100, ATGP0672-250, ATGP0672-500, ATGP0672-1

References & Citations

Tachikui., et al. (1997) M. J. Biochem. 121 (1) :145-9.; ; Gloria K., et al. (2006) Plant Physiology 141:1617-1629;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100 mM NaCl

Scientific Category

Cancer

NCBI Accession Number

NP_002892

Uniprot Accession Number

Q15293

Species

Human

AA Sequence

ELEK PTVRKERVVR PDSELGERPP EDNQSFQYDH EAFLGKEDSK TFDQLTPDES KERLGKIVDR IDNDGDGFVT TEELKTWIKR VQKRYIFDNV AKVWKDYDRD KDDKISWEEY KQATYGYYLG NPAEFHDSSD HHTFKKMLPR DERRFKAADL NGDLTATREE FTAFLHPEEF EHMKEIVVLE TLEDIDKNGD GFVDQDEYIA DMFSHEENGP EPDWVLSERE QFNEFRDLNK DGKLDKDEIR HWILPQDYDH AQAEARHLVY ESDKNKDEKL TKEEILENWN MFVGSQATNY GEDLTKNHDE L