Recombinant hiv-1 Gag p24 protein

The HIV1 p24 protein performs highly complex orchestrated tasks during the assembly, budding maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag p24, the major capsid protein of the HIV-1 virion, has been used in clinical trials as one of the components of the HIV-1 vaccine because of the high degree of sequence conservation between different isolates. Recombinant HIV1-p24 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Group-specific antigen - p24, CA, Capsid protein p24, Group-specific antigen - p24

Expression System

E.coli

Antigen Species

HIV-1

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

21.2 kDa (188aa) confirmed by MALDI-TOF

Additionnal Information

Gag p24, ATGP0671-10 µg, ATGP0671-20 µg, ATGP0671-50 µg, ATGP0671-100 µg, ATGP0671-250 µg, ATGP0671-500 µg, ATGP0671-1 mg, ATGP0671-10, ATGP0671-20, ATGP0671-50, ATGP0671-100, ATGP0671-250, ATGP0671-500, ATGP0671-1

References & Citations

Bernard NF., et al. (2009) Clin Immunol. 131 (2) :277-87.; ; Wagstaff M., et al. (1995) Immunology. 86 (1) :85-96.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 0.1mM PMSF, 10% glycerol

Scientific Category

Virus

NCBI Accession Number

AAA44987.1

Uniprot Accession Number

P12493

Species

HIV-1

AA Sequence

WVKVVEEKA FSPEVIPMFS ALSEGATPQD LNTMLNTVGG HQAAMQMLKE TINEEAAEWD RLHPVHAGPI APGQMREPRG SDIAGTTSTL QEQIGWMTHN PPIPVGEIYK RWIILGLNKI VRMYSPTSIL DIRQGPKEPF RDYVDRFYKT LRAEQASQEV KNWMTETL