Recombinant human DR1 protein
DR1, also known as NC2-beta, is a TBP (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. By selectively repressing polymerases II and III, DR1 may shift the physiological balance of transcriptional output in favor of polymerase I. Recombinant human DR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography
Product Specifications
Product Name Alternative
Down-regulator of transcription 1 TBP-binding, NC2, NC2-BETA, Down-regulator of transcription 1, TBP-binding
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
21.6 kDa (196aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
White RJ., et al. (2010) Nucleic Acids Res. 38 (4) :1228-39. ; ; Collart MA., et al. (2008) Nucleic Acids Res. 36 (2) :539-49.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 0.1mM PMSF ,10% glycerol
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001929.1
Uniprot Accession Number
Q01658
Species
Human
AA Sequence
Frequently Asked Questions
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