Recombinant human DR1 protein

DR1, also known as NC2-beta, is a TBP (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. By selectively repressing polymerases II and III, DR1 may shift the physiological balance of transcriptional output in favor of polymerase I. Recombinant human DR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography

Product Specifications

Product Name Alternative

Down-regulator of transcription 1 TBP-binding, NC2, NC2-BETA, Down-regulator of transcription 1, TBP-binding

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

21.6 kDa (196aa) confirmed by MALDI-TOF

Additionnal Information

DR1, Down-regulator of transcription 1 TBP-binding, NC2, NC2-BETA, Down-regulator of transcription 1, TBP-binding, ATGP0669-10 µg, ATGP0669-20 µg, ATGP0669-50 µg, ATGP0669-100 µg, ATGP0669-250 µg, ATGP0669-500 µg, ATGP0669-1 mg, ATGP0669-10, ATGP0669-20, ATGP0669-50, ATGP0669-100, ATGP0669-250, ATGP0669-500, ATGP0669-1

References & Citations

White RJ., et al. (2010) Nucleic Acids Res. 38 (4) :1228-39. ; ; Collart MA., et al. (2008) Nucleic Acids Res. 36 (2) :539-49.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 0.1mM PMSF ,10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001929.1

Uniprot Accession Number

Q01658

Species

Human

AA Sequence

MASSSGNDDD LTIPRAAINK MIKETLPNVR VANDARELVV NCCTEFIHLI SSEANEICNK SEKKTISPEH VIQALESLGF GSYISEVKEV LQECKTVALK RRKASSRLEN LGIPEEELLR QQQELFAKAR QQQAELAQQE WLQMQQAAQQ AQLAAASASA SNQAGSSQDE EDDDDI