Recombinant human OGG1 protein

OGG1, also known as 8-oxoguanine glycosylase, is a DNA glycosylase enzyme involved in base excision repair. This protein is the primary enzyme responsible for the excision of 7, 8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen species (ROS) . It has a beta lyase activity that nicks DNA 3' to the lesion. Recombinant human OGG1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

8-oxoguanine DNA glycosylase, N-glycosylase/DNA lyase, DNA-apurinic or apyrimidinic site lyase, AP lyase, HMMH, HOGG1, OGH1, MUTM

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

41.2 kDa (368aa) confirmed by MALDI-TOF

Additionnal Information

8-oxoGuanine DNA Glycosylase, OGG1, 8-oxoguanine DNA glycosylase 1, HMMH, HOGG1, MuTM, OGH1, AP lyase, 8-oxoguanine DNA glycosylase 1, ATGP0668-10 µg, ATGP0668-20 µg, ATGP0668-50 µg, ATGP0668-100 µg, ATGP0668-250 µg, ATGP0668-500 µg, ATGP0668-1 mg, ATGP0668-10, ATGP0668-20, ATGP0668-50, ATGP0668-100, ATGP0668-250, ATGP0668-500, ATGP0668-1

References & Citations

Seeberg E., et al. (2002) . Nucleic Acids Res. 30 (11) :2349-57.; ; Hodges NJ., et al (2010) . DNA Repair (Amst) . 9 (2) :144-52.;

Other References

Duan S, et al. Interaction between RECQL4 and OGG1 promotes repair of oxidative base lesion 8-oxoG and is regulated by SIRT1 deacetylase. (Nucleic Acids Res. 2020) {https://pubmed.ncbi.nlm.nih.gov/32432680/}; ; Schniertshauer D, et al. Real-time Base Excision Repair Assay to Measure the Activity of the 8-oxoguanine DNA Glycosylase 1 in Isolated Mitochondria of Human Skin Fibroblasts. (Bio Protoc. 2021) {https://pubmed.ncbi.nlm.nih.gov/33855116/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 40% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

AAH00657.1

Uniprot Accession Number

O15527

Species

Human

AA Sequence

MPARALL PRRMGHRTLA STPALWASIP CPRSELRLDL VLPSGQSFRW REQSPAHWSG VLADQVWTLT QTEEQLHCTV YRGDKSQASR PTPDELEAVR KYFQLDVTLA QLYHHWGSVD SHFQEVAQKF QGVRLLRQDP IECLFSFICS SNNNIARITG MVERLCQAFG PRLIQLDDVT YHGFPSLQAL AGPEVEAHLR KLGLGYRARY VSASARAILE EQGGLAWLQQ LRESSYEEAH KALCILPGVG TKVADCICLM ALDKPQAVPV DVHMWHIAQRDYSWHPTTSQ AKGPSPQTNK ELGNFFRSLW GPYAGWAQAV LFSADLRQCR HAQEPPAKRR KGSKGPEG