Recombinant human Malate dehydrogenase 1/MDH1 protein

MDH1 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. This protein also regulates p53-dependent cell-cycle arrest and apoptosis in response to glucose deprivation. Recombinant human MDH1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 250 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Product Name Alternative

Malate dehydrogenase cytoplasmic, MDH-s, MDHA, MOR2, Diiodophenylpyruvate reductase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 250unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Molecular Weight

37.4 kDa (342aa) confirmed by MALDI-TOF

Additionnal Information

MOR2, MDH-s, MDHA, MDH1, Malate dehydrogenase cytoplasmic, Malate dehydrogenase, Diiodophenylpyruvate reductase, cytoplasmic, ATGP0665-10 µg, ATGP0665-20 µg, ATGP0665-50 µg, ATGP0665-100 µg, ATGP0665-250 µg, ATGP0665-500 µg, ATGP0665-1 mg, ATGP0665-010, ATGP0665-020, ATGP0665-050, ATGP0665-100, ATGP0665-250, ATGP0665-500, ATGP0665-01M

References & Citations

Youn HD., et al. (2009) Cell Death Differ. 16 (5) :738-48; ; Waye MM., et al (2005) J Cell Biochem. 94 (4) :763-73.;

Other References

Yoshida T, et al. A covalent small molecule inhibitor of glutamate-oxaloacetate transaminase 1 impairs pancreatic cancer growth. (Biochem Biophys Res Commun. 2020) {https://pubmed.ncbi.nlm.nih.gov/31787239/}