Recombinant E.coli surA protein

SurA is a periplasmic peptidyl-prolyl isomerase (PPIase) and chaperone of Escherichia coli and other Gram-negative bacteria. This protein is a major factor in the biogenesis of beta-barrel outer membrane proteins (OMPs) and plays an integral role in cell envelope homeostasis and cell envelope functions. It is essential for the survival of E. coli in stationary phase and required for pilus biogenesis. Recombinant E. coli SurA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Product Name Alternative

Chaperone surA, PPIase surA, Rotamase surA, Survival protein A

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Molecular Weight

47.3 kDa (429aa) confirmed by MALDI-TOF

Additionnal Information

SurA, Chaperone surA, PPIase surA, Rotamase surA, Survival protein A, Chaperone surA, ATGP0664-10 µg, ATGP0664-20 µg, ATGP0664-50 µg, ATGP0664-100 µg, ATGP0664-250 µg, ATGP0664-500 µg, ATGP0664-1 mg, ATGP0664-10, ATGP0664-20, ATGP0664-50, ATGP0664-100, ATGP0664-250, ATGP0664-500, ATGP0664-1

References & Citations

Virlogeux-Payant I., et al (2009) Microbiology. 155 (Pt 5) :1613-22; ; Kolter R., et al. (1996) J Bacteriol. 178 (6) :1770-3.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_414595

Uniprot Accession Number

P0ABZ6

Species

E.coli

AA Sequence

MAPQVVDKVA AVVNNGVVLE SDVDGLMQSV KLNAAQARQQ LPDDATLRHQ IMERLIMDQI ILQMGQKMGV KISDEQLDQA IANIAKQNNM TLDQMRSRLA YDGLNYNTYR NQIRKEMIIS EVRNNEVRRR ITILPQEVES LAQQVGNQND ASTELNLSHI LIPLPENPTS DQVNEAESQA RAIVDQARNG ADFGKLAIAH SADQQALNGG QMGWGRIQEL PGIFAQALST AKKGDIVGPI RSGVGFHILK VNDLRGESKN ISVTEVHARH ILLKPSPIMT DEQARVKLEQ IAADIKSGKT TFAAAAKEFS QDPGSANQGG DLGWATPDIF DPAFRDALTR LNKGQMSAPV HSSFGWHLIE LLDTRNVDKT DAAQKDRAYR MLMNRKFSEE AASWMQEQRA SAYVKILSN