Recombinant E.coli surA protein
SurA is a periplasmic peptidyl-prolyl isomerase (PPIase) and chaperone of Escherichia coli and other Gram-negative bacteria. This protein is a major factor in the biogenesis of beta-barrel outer membrane proteins (OMPs) and plays an integral role in cell envelope homeostasis and cell envelope functions. It is essential for the survival of E. coli in stationary phase and required for pilus biogenesis. Recombinant E. coli SurA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Specifications
Product Name Alternative
Chaperone surA, PPIase surA, Rotamase surA, Survival protein A
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
47.3 kDa (429aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Virlogeux-Payant I., et al (2009) Microbiology. 155 (Pt 5) :1613-22; ; Kolter R., et al. (1996) J Bacteriol. 178 (6) :1770-3.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_414595
Uniprot Accession Number
P0ABZ6
Species
E.coli
AA Sequence
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