Recombinant human Fascin protein

FSCN1 is an actin-bundling protein that provides rigidity to filopodial bundles to efficiently push the membrane forward during cytoskeleton remodeling and cell migration. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers. And this protein is absent from most normal epithelia yet is upregulated in multiple forms of human carcinoma, where its expression correlates clinically with a poor prognosis. FSCN1 expression has been localized to neurons, glial cells, and endothelial cells. Recombinant human FSCN1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Fascin, FAN1, HSN, SNL, Fascin

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

56.7 kDa (513aa)

Additionnal Information

Fascin, Fascin, FAN1, HSN, SNL, Fascin, ATGP0662-10 µg, ATGP0662-20 µg, ATGP0662-50 µg, ATGP0662-100 µg, ATGP0662-250 µg, ATGP0662-500 µg, ATGP0662-1 mg, ATGP0662-10, ATGP0662-20, ATGP0662-50, ATGP0662-100, ATGP0662-250, ATGP0662-500, ATGP0662-1

References & Citations

Wu D, et al. (2010) Ann Oncol. 21 (3) :589-96; ; Shin H, et al. (2009) Phys Rev Left 103 (23) :238102;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100 mM NaCl

Scientific Category

Cytoskeleton

NCBI Accession Number

NP_003079

Uniprot Accession Number

Q16658

Species

Human

AA Sequence

MTANGTAEAV QIQFGLINCG NKYLTAEAFG FKVNASASSL KKKQIWTLEQ PPDEAGSAAV CLRSHLGRYL AADKDGNVTC EREVPGPDCR FLIVAHDDGR WSLQSEAHRR YFGGTEDRLS CFAQTVSPAE KWSVHIAMHP QVNIYSVTRK RYAHLSARPA DEIAVDRDVP WGVDSLITLA FQDQRYSVQT ADHRFLRHDG RLVARPEPAT GYTLEFRSGK VAFRDCEGRY LAPSGPSGTL KAGKATKVGK DELFALEQSC AQVVLQAANE RNVSTRQGMD LSANQDEETD QETFQLEIDR DTKKCAFRTH TGKYWTLTAT GGVQSTASSK NASCYFDIEW RDRRITLRAS NGKFVTSKKN GQLAASVETA GDSELFLMKL INRPIIVFRG EHGFIGCRKV TGTLDANRSS YDVFQLEFND GAYNIKDSTG KYWTVGSDSA VTSSGDTPVD FFFEFCDYNK VAIKVGGRYL KGDHAGVLKA SAETVDPASL WEY