Recombinant E.coli udp protein

Uridine phosphorypase (uDP) catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is uridine:phosphate alpha-D-ribosyltransferase. Other names in common use include pyrimidine phosphorylase, urdPase, uPH, and uPase. Recombinant E. coli uDP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of uridine presence of phosphate per minute at pH 7.5 at 25C.

Product Name Alternative

Uridine phosphorylase, UrdPase, Upase

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 20,000pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0pmole of uridine presence of phosphate per minute at pH 7.5 at 25C.

Molecular Weight

29.3 kDa (273aa) confirmed by MALDI-TOF

Additionnal Information

UDP, uridine phosphorylase, urdPase, upase, uridine phosphorylase, ATGP0661-10 µg, ATGP0661-20 µg, ATGP0661-50 µg, ATGP0661-100 µg, ATGP0661-250 µg, ATGP0661-500 µg, ATGP0661-1 mg, ATGP0661-10, ATGP0661-20, ATGP0661-50, ATGP0661-100, ATGP0661-250, ATGP0661-500, ATGP0661-1

References & Citations

Burling F.T., et al. (2003) Acta Crystallogr. 59:73-76; ; Walton L., et al (1989) Nucleic Acids Res. 17 (16) :6741.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_418275

Uniprot Accession Number

P12758

Species

E.coli

AA Sequence

MSKSDVFHLG LTKNDLQGAT LAIVPGDPDR VEKIAALMDK PVKLASHREF TTWRAELDGK PVIVCSTGIG GPSTSIAVEE LAQLGIRTFL RIGTTGAIQP HINVGDVLVT TASVRLDGAS LHFAPLEFPA VADFECTTAL VEAAKSIGAT THVGVTASSD TFYPGQERYD TYSGRVVRHF KGSMEEWQAM GVMNYEMESA TLLTMCASQG LRAGMVAGVI VNRTQQEIPN AETMKQTESH AVKIVVEAAR RLL