Recombinant human ARFL1/ARL1 protein
ADP-ribosylation factor-like protein 1 (ARL1) belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs) . ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Recombinant human ARL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
ARFL1, ADP-ribosylation factor-like protein 1, ADP ribosylation factor like GTPase 1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
22.5 kDa (201aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Nishimoto-Morita K., et al. (2009) J. Biol. Chem 284 (16) :10583-92; ; Van Valkenburgh H., et al. (2001) J. Biol. Chem. 276:22826-22837;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 100 mM NaCl, 2mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_001168
Uniprot Accession Number
P40616
Species
Human
AA Sequence
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