Recombinant human TSTA3 protein

TSTA3 is a NADP (H) -binding protein. It catalyzes the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II. Recombinant human TSTA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

GDP-L-fucose synthase, FX, P35B, SDR4E1, GDP-L-fucose synthase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

38.0 kDa (341aa) confirmed by MALDI-TOF

Additionnal Information

TSTA3, GDP-L-fucose synthase, FX, P35B, SDR4E1, GDP-L-fucose synthase, ATGP0657-10 µg, ATGP0657-20 µg, ATGP0657-50 µg, ATGP0657-100 µg, ATGP0657-250 µg, ATGP0657-500 µg, ATGP0657-1 mg, ATGP0657-10, ATGP0657-20, ATGP0657-50, ATGP0657-100, ATGP0657-250, ATGP0657-500, ATGP0657-1

References & Citations

Camardella L., et al. (1995) Blood 85 (1) : 264-7; ; Tonetti M., et al. (1996) J. Biol. Chem. 271:27274-27279;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 50 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_003304

Uniprot Accession Number

Q13630

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGEPQGSMRI LVTGGSGLVG KAIQKVVADG AGLPGEDWVF VSSKDADLTD TAQTRALFEK VQPTHVIHLA AMVGGLFRNI KYNLDFWRKN VHMNDNVLHS AFEVGARKVV SCLSTCIFPD KTTYPIDETM IHNGPPHNSN FGYSYAKRMI DVQNRAYFQQ YGCTFTAVIP TNVFGPHDNF NIEDGHVLPG LIHKVHLAKS SGSALTVWGT GNPRRQFIYS LDLAQLFIWV LREYNEVEPI ILSVGEEDEV SIKEAAEAVV EAMDFHGEVT FDTTKSDGQF KKTASNSKLR TYLPDFRFTP FKQAVKETCA WFTDNYEQAR K