Recombinant human TSTA3 protein
TSTA3 is a NADP (H) -binding protein. It catalyzes the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II. Recombinant human TSTA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
GDP-L-fucose synthase, FX, P35B, SDR4E1, GDP-L-fucose synthase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
38.0 kDa (341aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Camardella L., et al. (1995) Blood 85 (1) : 264-7; ; Tonetti M., et al. (1996) J. Biol. Chem. 271:27274-27279;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 50 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_003304
Uniprot Accession Number
Q13630
Species
Human
AA Sequence
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