Recombinant human EDF1 protein

Endothelial differentiation-related factor 1, also known as EDF1 may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. Also, EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas. Recombinant human EDF1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Endothelial differentiation-related factor 1, EDF-1, MBF1, Endothelial differentiation-related factor 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

17.4 kDa (156aa) confirmed by MALDI-TOF

Additionnal Information

EDF1, Endothelial differentiation-related factor 1, EDF-1, MBF1, Endothelial differentiation-related factor 1, ATGP0655-10 µg, ATGP0655-20 µg, ATGP0655-50 µg, ATGP0655-100 µg, ATGP0655-250 µg, ATGP0655-500 µg, ATGP0655-1 mg, ATGP0655-10, ATGP0655-20, ATGP0655-50, ATGP0655-100, ATGP0655-250, ATGP0655-500, ATGP0655-1

References & Citations

Mariotti M., et al. (2000) J Biol Chem. 275:24047-24051.; ; Dragoni I., et al. (1998) J Biol Chem. 273: 31119-31124. ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_003783

Uniprot Accession Number

O60869

Species

Human

AA Sequence

MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK QHSITKNTAK LDRETEELHH DRVTLEVGKV IQQGRQSKGL TQKDLATKIN EKPQVIADYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPRAKLE HHHHHH