Recombinant human PDXP protein

Pyridoxal phosphate phosphatase, also known as PDXP is the enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. PDXP catalyzes the dephosphorylation of pyridoxal 5'-phosphate and exhibits a high level of expression various parts of the central nervous system, especially the brain. PDXP activity is catalyzed by haloacid dehalogenase (HAD), and it is the cofactor of both aromatic L-amino acid decarboxylase and glutamate decarboxylase. Recombinant human PDXP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Pyridoxal phosphate phosphatase, CIN, PLP, PLPP, Pyridoxal phosphate phosphatase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

33.8 kDa (316aa) confirmed by MALDI-TOF

Additionnal Information

PDXP, Pyridoxal phosphate phosphatase, CIN, PLP, PLPP, Pyridoxal phosphate phosphatase, ATGP0652-10 µg, ATGP0652-20 µg, ATGP0652-50 µg, ATGP0652-100 µg, ATGP0652-250 µg, ATGP0652-500 µg, ATGP0652-1 mg, ATGP0652-10, ATGP0652-20, ATGP0652-50, ATGP0652-100, ATGP0652-250, ATGP0652-500, ATGP0652-1

References & Citations

Jang YM., et al. (1992) J Biol Chem. 278 (50) :50040-6.; ; Fonda ML., et al. (1992) J Biol Chem. 267 (22) :15978-83.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_064711

Uniprot Accession Number

Q96GD0

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MARCERLRGA ALRDVLGRAQ GVLFDCDGVL WNGERAVPGA PELLERLARA GKAALFVSNN SRRARPELAL RFARLGFGGL RAEQLFSSAL CAARLLRQRL PGPPDAPGAV FVLGGEGLRA ELRAAGLRLA GDPSAGDGAA PRVRAVLVGY DEHFSFAKLR EACAHLRDPE CLLVATDRDP WHPLSDGSRT PGTGSLAAAV ETASGRQALV VGKPSPYMFE CITENFSIDP ARTLMVGDRL ETDILFGHRC GMTTVLTLTG VSRLEEAQAY LAAGQHDLVP HYYVESIADL TEGLED