Recombinant human RND3 protein
Rho-related GTP-binding protein RhoE, also known as RND3, is a small GTPase of the Ras superfamily that regulate the organization of the actin cytoskeleton in response to extracellular growth factors. RND3 activity leads to a decrease in stress fibers and increased cell migration, at least in part through regulation of the Rho-dependent kinase ROCK1. Also, RND3 has been implicated in inhibition of DNA-damage induced apoptosis and cell cycle arrest. Recombinant human RND3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Rho-related GTP-binding protein RhoE, ARHE, memB, Rho8, RhoE, Rho-related GTP-binding protein RhoE
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
29.2 kDa (261aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Riento K., et al. (2005) Mol Cell Biol. 23:4219-29.; ; Boswell S A., et al. (2007) J Biol Chem. 282:4850-8;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 1mM EDTA, 0.1M NaCl
Scientific Category
Cancer
NCBI Accession Number
NP_005159
Uniprot Accession Number
P61587
Species
Human
AA Sequence
Frequently Asked Questions
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