Recombinant human RND3 protein

Rho-related GTP-binding protein RhoE, also known as RND3, is a small GTPase of the Ras superfamily that regulate the organization of the actin cytoskeleton in response to extracellular growth factors. RND3 activity leads to a decrease in stress fibers and increased cell migration, at least in part through regulation of the Rho-dependent kinase ROCK1. Also, RND3 has been implicated in inhibition of DNA-damage induced apoptosis and cell cycle arrest. Recombinant human RND3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Rho-related GTP-binding protein RhoE, ARHE, memB, Rho8, RhoE, Rho-related GTP-binding protein RhoE

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

29.2 kDa (261aa) confirmed by MALDI-TOF

Additionnal Information

RND3, Rho-related GTP-binding protein RhoE, ARHE, memB, Rho8, RhoE, Rho-related GTP-binding protein RhoE, ATGP0651-10 µg, ATGP0651-20 µg, ATGP0651-50 µg, ATGP0651-100 µg, ATGP0651-250 µg, ATGP0651-500 µg, ATGP0651-1 mg, ATGP0651-10, ATGP0651-20, ATGP0651-50, ATGP0651-100, ATGP0651-250, ATGP0651-500, ATGP0651-1

References & Citations

Riento K., et al. (2005) Mol Cell Biol. 23:4219-29.; ; Boswell S A., et al. (2007) J Biol Chem. 282:4850-8;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 1mM EDTA, 0.1M NaCl

Scientific Category

Cancer

NCBI Accession Number

NP_005159

Uniprot Accession Number

P61587

Species

Human

AA Sequence

MKERRASQKL SSKSIMDPNQ NVKCKIVVVG DSQCGKTALL HVFAKDCFPE NYVPTVFENY TASFEIDTQR IELSLWDTSG SPYYDNVRPL SYPDSDAVLI CFDISRPETL DSVLKKWKGE IQEFCPNTKM LLVGCKSDLR TDVSTLVELS NHRQTPVSYD QGANMAKQIG AATYIECSAL QSENSVRDIF HVATLACVNK TNKNVKRNKS QRATKRISHM PSRPELSAVA TDLRKDKAKS C