Recombinant human Sirtuin 3/SIRT3 protein

NAD-dependent deacetylase sirtuin-3, mitochondrial, also known as SIRT3, belongs to the sirtuin family of proteins. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance and metabolic regulation. SIRT3 exhibits NAD+-dependent deacetylase activity in the mitochondria. Over-expression of SIRT3 results in increased levels of the mitochondrial uncoupling protein 1. SIRT3 protein levels are also elevated in certain breast cancers. Recombinant human SIRT3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

NAD-dependent protein deacetylase sirtuin-3 mitochondrial, hSIRT3, Regulatory protein SIR2 homolog 3, SIR2-like protein 3, SIR2L3

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

33.5 kDa (303aa) confirmed by MALDI-TOF

Additionnal Information

Sirtulin3, SIRT3, NAD-dependent deacetylase sirtuin-3 mitochondrial, SIR2L3, Sirtuin3, NAD-dependent deacetylase sirtuin-3, mitochondrial, ATGP0650-10 µg, ATGP0650-20 µg, ATGP0650-50 µg, ATGP0650-100 µg, ATGP0650-250 µg, ATGP0650-500 µg, ATGP0650-1 mg, ATGP0650-10, ATGP0650-20, ATGP0650-50, ATGP0650-100, ATGP0650-250, ATGP0650-500, ATGP0650-1

References & Citations

Schewr B., et al. (2002) J Cell Biol. 158 (4) :647-57.; ; Kim H S., et al. (2010) Cancer Cell. 17 (1) :41-52.;

Other References

Kawaguchi M, et al. Development of Peptide-Based Sirtuin Defatty-Acylase Inhibitors Identified by the Fluorescence Probe, SFP3, That Can Efficiently Measure Defatty-Acylase Activity of Sirtuin. (J Med Chem. 2019) {https://pubmed.ncbi.nlm.nih.gov/31117516/}; ; Kawaguchi M, et al. A Fluorescent Probe for Imaging Sirtuin Activity in Living Cells, Based on One-Step Cleavage of the Dabcyl Quencher. (Chembiochem. 2016) {https://pubmed.ncbi.nlm.nih.gov/27542094/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Cancer

NCBI Accession Number

NP_036371.1

Uniprot Accession Number

Q9NTG7

Species

Human

AA Sequence

SDKGKLSLQ DVAELIRARA CQRVVVMVGA GISTPSGIPD FRSPGSGLYS NLQQYDLPYP EAIFELPFFF HNPKPFFTLA KELYPGNYKP NVTHYFLRLL HDKGLLLRLY TQNIDGLERV SGIPASKLVE AHGTFASATC TVCQRPFPGE DIRADVMADR VPRCPVCTGV VKPDIVFFGE PLPQRFLLHV VDFPMADLLL ILGTSLEVEP FASLTEAVRS SVPRLLINRD LVGPLAWHPR SRDVAQLGDV VHGVESLVEL LGWTEEMRDL VQRETGKLDG PDK