Recombinant E.coli nfsB protein
NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5- (aziridin-1-yl) -2, 4-dinitrobenzamide) . The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E. coli NFNB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Oxygen-insensitive NAD (P) H nitroreductase, Dihydropteridine reductase, FMN-dependent nitroreductase, dprA, nfnB
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
26.0 kDa (237aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Jarrom D., et al. (2009) Biochemistry. 48 (32) :7665-72. ; ; Singleton DC., et al. (2007) Cancer Gene Ther. 14 (12) :953-67.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM Nacl, 1mM DTT, 10% glycerol
Scientific Category
Redox Proteins
NCBI Accession Number
NP_415110
Uniprot Accession Number
P38489
Species
E.coli
AA Sequence
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