Recombinant E.coli nfsB protein

NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5- (aziridin-1-yl) -2, 4-dinitrobenzamide) . The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E. coli NFNB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Oxygen-insensitive NAD (P) H nitroreductase, Dihydropteridine reductase, FMN-dependent nitroreductase, dprA, nfnB

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

26.0 kDa (237aa) confirmed by MALDI-TOF

Additionnal Information

NFNB, dihydropteridine reductase NAD (P) H-dependent oxygen-insensitive, dprA, nfsB, nfsI, ntr, dihydropteridine reductase, NAD (P) H-dependent, oxygen-insensitive, ATGP0648-10 µg, ATGP0648-20 µg, ATGP0648-50 µg, ATGP0648-100 µg, ATGP0648-250 µg, ATGP0648-500 µg, ATGP0648-1 mg, ATGP0648-10, ATGP0648-20, ATGP0648-50, ATGP0648-100, ATGP0648-250, ATGP0648-500, ATGP0648-1

References & Citations

Jarrom D., et al. (2009) Biochemistry. 48 (32) :7665-72. ; ; Singleton DC., et al. (2007) Cancer Gene Ther. 14 (12) :953-67.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50 mM Nacl, 1mM DTT, 10% glycerol

Scientific Category

Redox Proteins

NCBI Accession Number

NP_415110

Uniprot Accession Number

P38489

Species

E.coli

AA Sequence

MDIISVALKR HSTKAFDASK KLTPEQAEQI KTLLQYSPSS TNSQPWHFIV ASTEEGKARV AKSAAGNYVF NERKMLDASH VVVFCAKTAM DDVWLKLVVD QEDADGRFAT PEAKAANDKG RKFFADMHRK DLHDDAEWMA KQVYLNVGNF LLGVAALGLD AVPIEGFDAA ILDAEFGLKE KGYTSLVVVP VGHHSVEDFN ATLPKSRLPQ NITLTEV