Recombinant human HIF-1 alpha/HIF1A protein

HIF1A, identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. This protein is a heterodimer composed of HIF-1beta subunit and one of three subunits (HIF-1A, HIF-2A or HIF-3A) . The activation of HIF1A is closely associated with a variety of tumors and oncogenic pathways. HIF1A consists of DNA binding domain (DBD domain), Dimerization domain and C-terminla regulatiory domains, including two transactivation domains (TAD), an oxygen-dependent degradation (ODD) domain, and inhibitory domains. Recombinant human HIF1A (576-785) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Hypoxia inducible factor 1 subunit alpha, Hypoxia-inducible factor 1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, PASD8, MOP1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

25.1 kDa (231aa) confirmed by MALDI-TOF (Real Molecular weight on SDS-PAGE will be shift up)

Additionnal Information

HIF1 a, Hypoxia-inducible factor 1-alpha, BHLHE78, MOP1, PASD8, Hypoxia-inducible factor 1-alpha, ATGP0647-10 µg, ATGP0647-20 µg, ATGP0647-50 µg, ATGP0647-100 µg, ATGP0647-250 µg, ATGP0647-500 µg, ATGP0647-1 mg, ATGP0647-10, ATGP0647-20, ATGP0647-50, ATGP0647-100, ATGP0647-250, ATGP0647-500, ATGP0647-1

References & Citations

Okuyama H. et al., (2006) J. Biol Chem. 281 (22) :15554-63

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001521

Uniprot Accession Number

Q16665

Species

Human

AA Sequence

MSFDQLSPLE SSSASPESAS PQSTVTVFQQ TQIQEPTANA TTTTATTDEL KTVTKDRMED IKILIASPSP THIHKETTSA TSSPYRDTQS RTASPNRAGK GVIEQTEKSH PRSPNVLSVA LSQRTTVPEE ELNPKILALQ NAQRKRKMEH DGSLFQAVGI GTLLQQPDDH AATTSLSWKR VKGCKSSEQN GMEQKTIILI PSDLACRLLG Q