Recombinant human Tau 352 (0N3R) /MAPT protein

MAPT, also known as TAu, is a neuronal microtubule associated protein found predominantly on axons. The function of this protein is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. MAPT, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Recombinant human MAPT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Microtubule-associated protein isoform 4, fetal tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, G protein beta1/gamma2 subunit-interacting factor 1, protein phosphatase 1 regulatory subunit 103, MTBT1, PPND, FTDP-17, TAU, MSTD, MTBT2, FLJ31424, MGC138549, PPP1R103, tau-40, DDPAC, MAPTL

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by BCA assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

38.9 kDa (372aa) confirmed by MALDI-TOF (Real Molecular weight on SDS-PAGE will be shift up)

Additionnal Information

Tau, Microtubule-associated protein tau, TAu, DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, Microtubule-associated protein tau, ATGP0646-10 µg, ATGP0646-20 µg, ATGP0646-50 µg, ATGP0646-100 µg, ATGP0646-250 µg, ATGP0646-500 µg, ATGP0646-1 mg, ATGP0646-10, ATGP0646-20, ATGP0646-50, ATGP0646-100, ATGP0646-250, ATGP0646-500, ATGP0646-1

References & Citations

Jack CR Jr., et al. (2010) Lancet Neurol. 9 (1) :119-28.; ; Buescher JL., et al. (2010) J Biol Chem. 285 (11) :7957-63.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M Nacl, 1mM DTT, 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_058525

Uniprot Accession Number

P10636

Species

Human

AA Sequence

MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKAEEAGI GDTPSLEDEA AGHVTQARMV SKSKDGTGSD DKKAKGADGK TKIATPRGAA PPGQKGQANA TRIPAKTPPA PKTPPSSGEP PKSGDRSGYS SPGSPGTPGS RSRTPSLPTP PTREPKKVAV VRTPPKSPSS AKSRLQTAPV PMPDLKNVKS KIGSTENLKH QPGGGKVQIV YKPVDLSKVT SKCGSLGNIH HKPGGGQVEV KSEKLDFKDR VQSKIGSLDN ITHVPGGGNK KIETHKLTFR ENAKAKTDHG AEIVYKSPVV SGDTSPRHLS NVSSTGSIDM VDSPQLATLA DEVSASLAKQ GL