Recombinant human RAD1 protein
RAD1 is a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. This complex also contains the Rad9 and Hus1 proteins and is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Recombinant human RAD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cell cycle checkpoint protein RAD1, HRAD1, REC1, Cell cycle checkpoint protein RAD1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
33.9 kDa (302aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Liu Y., et al. (2010) J Biol Chem. 285 (8) :5974-82.; ; Yazinski SA., et al. (2009) Proc Natl Acad Sci u S A. 106 (50) :21282-7.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M Nacl, 1mM DTT, 10% glycerol
Scientific Category
Cell Cycle
NCBI Accession Number
NP_002844.1
Uniprot Accession Number
O60671
Species
Human
AA Sequence
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