Recombinant human RAD1 protein

RAD1 is a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. This complex also contains the Rad9 and Hus1 proteins and is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Recombinant human RAD1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cell cycle checkpoint protein RAD1, HRAD1, REC1, Cell cycle checkpoint protein RAD1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

33.9 kDa (302aa) confirmed by MALDI-TOF

Additionnal Information

RAD1, Cell cycle checkpoint protein RAD1, HRAD1, REC1, Cell cycle checkpoint protein RAD1, ATGP0645-10 µg, ATGP0645-20 µg, ATGP0645-50 µg, ATGP0645-100 µg, ATGP0645-250 µg, ATGP0645-500 µg, ATGP0645-1 mg, ATGP0645-10, ATGP0645-20, ATGP0645-50, ATGP0645-100, ATGP0645-250, ATGP0645-500, ATGP0645-1

References & Citations

Liu Y., et al. (2010) J Biol Chem. 285 (8) :5974-82.; ; Yazinski SA., et al. (2009) Proc Natl Acad Sci u S A. 106 (50) :21282-7.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M Nacl, 1mM DTT, 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_002844.1

Uniprot Accession Number

O60671

Species

Human

AA Sequence

MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQS EGLREAFSEL DMTSEVLQIT MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES