Recombinant human ARF1 protein

ARF1, also known as ADP-ribosylation factor 1, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. It is essential and ubiquitous in eukaryotes, as this protein is involved in vesicular transport and functioning via phospholipase D activation. ARF1 plays a role in membrane traffic and organelle integrity and are intimately tied to their reversible association with membranes and distinct interactions with membrane phospholipids. Recombinant human ARF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

ADP-ribosylation factor 1, ADP ribosylation factor 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

22.8 kDa (201aa) confirmed by MALDI-TOF

Additionnal Information

ARF1, ATGP0644-10 µg, ATGP0644-20 µg, ATGP0644-50 µg, ATGP0644-100 µg, ATGP0644-250 µg, ATGP0644-500 µg, ATGP0644-1 mg, ATGP0644-10, ATGP0644-20, ATGP0644-50, ATGP0644-100, ATGP0644-250, ATGP0644-500, ATGP0644-1

References & Citations

Lee CM., et al. (1992) J Biol Chem. 267 (13) :9028-34.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol 0.1M NaCl,1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001649

Uniprot Accession Number

P84077

Species

Human

AA Sequence

MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K