Recombinant human MMS2/UBE2V2 protein
UBE2V2, also known as ubiquitin-conjugating enzyme E2 variant 2, constitutes a distinct subfamily within the E2 protein family. They have sequence similarity to other ubiquitin-conjugating enzymes but lack the conserved cysteine residue that is critical for the catalytic activity of E2s. This protein also shares homology with ubiquitin-conjugating enzyme E2 variant 1 and yeast MMS2 gene product. It may be involved in the differentiation of monocytes and enterocytes. Recombinant human UBE2V2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
18.5 kDa (165aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Unk I., et al. (2008) Proc Natl Acad Sci u S A. 105 (10) :3768-73.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
Scientific Category
Ubiquitination
NCBI Accession Number
NP_003341
Uniprot Accession Number
Q15819
Species
Human
AA Sequence
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