Recombinant human ITPA protein

ITPA, also known as Inosine triphosphate pyrophosphatase, is an enzyme that catalyzes the pyrophosphohydrolysis of both ITP (inosine triphosphate) and dITP (deoxyinosine triphosphate) to IMP (inosine monophosphate) and diphosphate. IMP can be used as a substrate for purine nucleotide pathways. IMP can be phosphorylated to ITP, and ITPA can regulate the concentration of ITP in the cell by converting ITP back to IMP. Defects in ITPA result in ITPA deficiency which is thought to be inherited and is characterized by an over-accumulation of ITP in erythocytes, leukocytes and fibroblasts. Recombinant human ITPA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Inosine triphosphate pyrophosphatase, C20orf37, dJ794I6.3, HLC14-06-P, ITPase, My049, OK/SW-cl.9, Inosine triphosphate pyrophosphatase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

23.7 kDa (215aa) confirmed by MALDI-TOF

Additionnal Information

ITPA, Inosine triphosphate pyrophosphatase, C20orf37, dJ794I6.3, HLC14-06-P, ITPase, My049, OK/SW-cl.9, Inosine triphosphate pyrophosphatase, ATGP0639-10 µg, ATGP0639-20 µg, ATGP0639-50 µg, ATGP0639-100 µg, ATGP0639-250 µg, ATGP0639-500 µg, ATGP0639-1 mg, ATGP0639-10, ATGP0639-20, ATGP0639-50, ATGP0639-100, ATGP0639-250, ATGP0639-500, ATGP0639-1

References & Citations

Clawson GA.. et al. (1991) Cell Growth Differ. 2 (11) :575-82.; ; Lin S., et al. (2001) J Biol Chem.. 276 (22) :18695-701.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_258412

Uniprot Accession Number

Q9BY32

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MMAASLVGKK IVFVTGNAKK LEEVVQILGD KFPCTLVAQK IDLPEYQGEP DEISIQKCQE AVRQVQGPVL VEDTCLCFNA LGGLPGPYIK WFLEKLKPEG LHQLLAGFED KSAYALCTFA LSTGDPSQPV RLFRGRTSGR IVAPRGCQDF GWDPCFQPDG YEQTYAEMPK AEKNAVSHRF RALLELQEYF GSLAA