Recombinant human PPAR gamma/NR1C3 protein

PPARG, also known as Peroxisome proliferator-activated receptor gamma, is member of the nuclear hormone receptor subfamily of transcription factors. This protein regulates transcription of genes involved in Insulin action, adipocyte differentiation, lipid metabolism and inflammation. It is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. It also activators include prostanoids, fatty acids, thiazolidinediones and N- (2-benzoylphenyl) tyrosine analogues. PPARG may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake. Recombinant human PPARG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Peroxisome proliferator-activated receptor gamma, NR1C3, CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARgamma, Peroxisome proliferator-activated receptor gamma

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

33 kDa (290aa) confirmed by MALDI-TOF

Additionnal Information

PPAR g, NR1C3, Peroxisome proliferator-activated receptor gamma, NR1C3, CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARgamma, Peroxisome proliferator-activated receptor gamma, ATGP0637-10 µg, ATGP0637-20 µg, ATGP0637-50 µg, ATGP0637-100 µg, ATGP0637-250 µg, ATGP0637-500 µg, ATGP0637-1 mg, ATGP0637-10, ATGP0637-20, ATGP0637-50, ATGP0637-100, ATGP0637-250, ATGP0637-500, ATGP0637-1

References & Citations

Berger J.. et al. (2002) Annu Rev Med . 53:409-35.; ; Michalik L ., et al. (2006) Pharmacol Rev. 58 (4) :726-41.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_619725.2

Uniprot Accession Number

P37231

Species

Human

AA Sequence

ADLRALAKH LYDSYIKSFP LTKAKARAIL TGKTTDKSPF VIYDMNSLMM GEDKIKFKHI TPLQEQSKEV AIRIFQGCQF RSVEAVQEIT EYAKSIPGFV NLDLNDQVTL LKYGVHEIIY TMLASLMNKD GVLISEGQGF MTREFLKSLR KPFGDFMEPK FEFAVKFNAL ELDDSDLAIF IAVIILSGDR PGLLNVKPIE DIQDNLLQAL ELQLKLNHPE SSQLFAKLLQ KMTDLRQIVT EHVQLLQVIK KTETDMSLHP LLQEIYKDLY